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Range (%) Average recovery (%) Serum (n = 8) 85-101 97 Plasma (n = 8) 92-105 102 Cell culture supernatant (n = 8) 96-108 105
It exerts its biological effects by promoting the production of pro-inflammatory cytokines
Substitute the OD450 value of the sample into the standard curve to calculate the concentration value of the sample
As controls
Rat ES ELISA Kit Growth Factor Family Range (%) Average recovery (%)Product Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20