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and a dinucleotide 3' overhang
Academi c Press
RNase III cleaves long double-stranded RNA (dsRNA) into short 12-30 base dsRNAs containing a 5'-PO4
[3] Morais
10X Capping Buffer GMP level:Non-GMP and a dinucleotide 3' overhangThe 10X Capping Buffer is a buffer solution used in conjunction with the Faustovirus capping enzyme, Vaccinia Capping Enzyme, mRNA Cap 2' O Methyltransferase. Related Buffer: S adenosylmethionine (SAM) Inside with: 500mM Tris HCl, pH 8. 0, 50mM KCl, 10mM MgCl2, 10mM DTT. Recommended Storage: 20 For detailed oprating instructions, please refer to the enzyme manual.
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